期刊
NANOMATERIALS
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/nano11081952
关键词
bacterial nanocellulose; small-diameter vascular grafts; endothelialization; tissue engineering; bioreactor
类别
资金
- German Research Foundation [WA 4489/1-1]
This study contrasted the effects of different surface coatings on the growth of human cells on bacterial nanocellulose small-diameter vascular grafts. It was found that fibronectin and heparin-chitosan coatings could positively impact endothelialization, potentially improving the longevity and reducing thrombogenicity of the vascular grafts in the future.
In this study, we contrast the impacts of surface coating bacterial nanocellulose small-diameter vascular grafts (BNC-SDVGs) with human albumin, fibronectin, or heparin-chitosan upon endothelialization with human saphenous vein endothelial cells (VEC) or endothelial progenitor cells (EPC) in vitro. In one scenario, coated grafts were cut into 2D circular patches for static colonization of a defined inner surface area; in another scenario, they were mounted on a customized bioreactor and subsequently perfused for cell seeding. We evaluated the colonization by emerging metabolic activity and the preservation of endothelial functionality by water soluble tetrazolium salts (WST-1), acetylated low-density lipoprotein (AcLDL) uptake assays, and immune fluorescence staining. Uncoated BNC scaffolds served as controls. The fibronectin coating significantly promoted adhesion and growth of VECs and EPCs, while albumin only promoted adhesion of VECs, but here, the cells were functionally impaired as indicated by missing AcLDL uptake. The heparin-chitosan coating led to significantly improved adhesion of EPCs, but not VECs. In summary, both fibronectin and heparin-chitosan coatings could beneficially impact the endothelialization of BNC-SDVGs and might therefore represent promising approaches to help improve the longevity and reduce the thrombogenicity of BNC-SDVGs in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据