4.4 Article

Metabolic profiling analysis of the vitamin B12 producer Propionibacterium freudenreichii

期刊

MICROBIOLOGYOPEN
卷 10, 期 3, 页码 -

出版社

WILEY
DOI: 10.1002/mbo3.1199

关键词

medium optimization; metabolomics; Propionibacterium freudenreichii; vitamin B-12 production

资金

  1. Science and Technology Service Network (STS) Initiative of the Chinese Academy of Sciences (CAS) [KFJ-STS-ZDTP-065]
  2. Tianjin Science Fund for Distinguished Young Scholars [17JCJQJC45300]
  3. National Key R&D Program of China [2018YFA0900300]

向作者/读者索取更多资源

This study found that glucose, yeast extract, KH2PO4, and glycine have significant effects on VB12 production by Propionibacterium freudenreichii. By optimizing the culture medium, the VB12 titer was significantly increased, and metabolomics analysis demonstrated that the optimized medium enhanced the synthesis of cellular metabolites, leading to improved VB12 production.
Vitamin B-12 (VB12) is an indispensable cofactor of metabolic enzymes and has been widely used in the food and pharmaceutical industries. In this study, the effects of medium composition on VB12 production by Propionibacterium freudenreichii were evaluated and optimized based on statistical experiments. The results showed that glucose, yeast extract, KH2PO4, and glycine have significant effects on VB12 production. The final titer of VB12 reached 8.32 +/- 0.02 mg/L, representing a 120% increase over the non-optimized culture medium. We employed a metabolomics approach to analyze the differences of metabolite concentrations in P. freudenreichii cells cultivated in the original medium and optimized fermentation medium. Using multivariate data analysis, we identified a range of correlated metabolites, illustrating how metabolomics can be used to explain VB12 production changes by corresponding differences in the overall cellular metabolism. The concentrations of many metabolic intermediates of glycolysis, the Wood-Werkman cycle, the TCA cycle, and amino acid metabolism were increased, which contributed to the synthesis of propionic acid and VB12 due to an improved supply of energy and precursors.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据