期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 174, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/62980
关键词
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资金
- National Science Foundation Graduate Research Fellowship Program (NSF-GRFP) [DGE - 1644869]
- National Eye Institute (NEI) [5T32EY013933]
- National Institute on Aging (NIA) [R21AG063012]
This report provides a detailed method for using RVO mouse models to study hypoxic-ischemic injury in the neural retina, with recommendations for optimizing specific steps to achieve consistently successful occlusion rates. Adjusting the wait time after rose bengal administration and balancing laser output can limit experimental variability and improve occlusion success rate.
Mouse models of retinal vein occlusion (RVO) are often used in ophthalmology to study hypoxic-ischemic injury in the neural retina. In this report, a detailed method pointing out critical steps is provided with recommendations for optimization to achieve consistently successful occlusion rates across different genetically modified mouse strains. The RVO mouse model consists primarily of the intravenous administration of a photosensitizer dye followed by laser photocoagulation using a retinal imaging microscope attached to an ophthalmic guided laser. Three variables were identified as determinants of occlusion consistency. By adjusting the wait time after rose bengal administration and balancing the baseline and experimental laser output, the variability across experiments can be limited and a higher success rate of occlusions achieved. This method can be used to study retinal diseases that are characterized by retinal edema and hypoxic-ischemic injury. Additionally, as this model induces vascular injury, it can also be applied to study the neurovasculature, neuronal death, and inflammation.
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