期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 174, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/62546
关键词
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资金
- WaNPRC/ITHS [P51OD010425]
- National Institute of Health (NIH) [EY023277, EY030441, MH114126, EY028902]
- NIH [OD010425]
- University of Washington Royalty Research Fund [A148416]
The method described in the text involves using injection cannulas made from polytetrafluoroethylene and stainless-steel tubes, which are autoclavable, disposable, and have low minimal-loading volumes. An inert, red-dyed mineral oil is used to fill the dead space, allowing for precise measurement of injection rates and volumes. This procedure can produce robust expression of optogenetic proteins in non-human primates for optical control of neural activity and behavior.
Optogenetic techniques have revolutionized neuroscience research and are poised to do the same for neurological gene therapy. The clinical use of optogenetics, however, requires that safety and efficacy be demonstrated in animal models, ideally in non-human primates (NHPs), because of their neurological similarity to humans. The number of candidate vectors that are potentially useful for neuroscience and medicine is vast, and no high-throughput means to test these vectors yet exists. Thus, there is a need for techniques to make multiple spatially and volumetrically accurate injections of viral vectors into NHP brain that can be identified unambiguously through postmortem histology. Described herein is such a method. Injection cannulas are constructed from coupled polytetrafluoroethylene and stainless-steel tubes. These cannulas are autoclavable, disposable, and have low minimal-loading volumes, making them ideal for the injection of expensive, highly concentrated viral vector solutions. An inert, red-dyed mineral oil fills the dead space and forms a visible meniscus with the vector solution, allowing instantaneous and accurate measurement of injection rates and volumes. The oil is loaded into the rear of the cannula, reducing the risk of co-injection with the vector. Cannulas can be loaded in 10 min, and injections can be made in 20 min. This procedure is well suited for injections into awake or anesthetized animals. When used to deliver high-quality viral vectors, this procedure can produce robust expression of optogenetic proteins, allowing optical control of neural activity and behavior in NHPs.
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