期刊
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
卷 12, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s40104-021-00607-w
关键词
In ovo feeding; L-gulonolactone oxidase; Sodium-dependent vitamin C transporter; Vitamin C; Yolk
资金
- National Key Research and Development Program of China [2017YFD0500500, 20170502200]
- National Natural Science Foundation of China [31972529]
- Innovative Transforming Project of Shaanxi Agricultural Achievements in Science Technology [NYKJ-2018-YL15]
- Program for Shaanxi Science & Technology from Shaanxi Provincial Science and Technology Department [2021TD-30]
This study found that the kidney and yolk sac are the main organs for VC synthesis in embryos, with the expression of the VC transporter SVCT1 gradually increasing during embryonic development. Plasma VC content was highest on the first day post-hatch. In ovo feeding of VC significantly increased VC content in plasma, brain, kidney, and liver, while also reducing embryonic mortality and increasing hatchability.
Background Many researches about in ovo feeding (IOF) of vitamin C (VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems. Results Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase (GLO) expression was lower during pre-hatch development than that during post-hatch development. Sodium-dependent vitamin C transporter 1 (SVCT1) expression was increased continuously in yolk sac from embryonic age 19 (E19) to post-hatch day 1 (D1) and in intestine (duodenum, jejunum and ileum) from E17 to D1. Plasma VC content was higher at D1 than that at D21 and D42. IOF of VC significantly reduced GLO expression in liver, kidney and yolk sac as well as SVCT1 expression in duodenum, jejunum and ileum, but increased the VC content in plasma, brain, kidney and liver. In addition, IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress. Conclusions This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation. These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.
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