期刊
GENES
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/genes12060797
关键词
genome editing; CRISPR; base editing; prime editing; DNA-free genome editing; crop improvement
资金
- Ministry of Education, Youth and Sports of the Czech Republic
Genome-editing technology, particularly the transgene-free approach, has great potential in achieving global food security. Recent developments in CRISPR-based tools for crop improvement include the development of transgene-free genome plants, compatible nucleases, and safe delivery vehicles. Despite the promising advancements, challenges remain in efficient delivery of CRISPR reagents, precision genome editing, and navigating government policies and public acceptance.
Genome-editing (GE) is having a tremendous influence around the globe in the life science community. Among its versatile uses, the desired modifications of genes, and more importantly the transgene (DNA)-free approach to develop genetically modified organism (GMO), are of special interest. The recent and rapid developments in genome-editing technology have given rise to hopes to achieve global food security in a sustainable manner. We here discuss recent developments in CRISPR-based genome-editing tools for crop improvement concerning adaptation, opportunities, and challenges. Some of the notable advances highlighted here include the development of transgene (DNA)-free genome plants, the availability of compatible nucleases, and the development of safe and effective CRISPR delivery vehicles for plant genome editing, multi-gene targeting and complex genome editing, base editing and prime editing to achieve more complex genetic engineering. Additionally, new avenues that facilitate fine-tuning plant gene regulation have also been addressed. In spite of the tremendous potential of CRISPR and other gene editing tools, major challenges remain. Some of the challenges are related to the practical advances required for the efficient delivery of CRISPR reagents and for precision genome editing, while others come from government policies and public acceptance. This review will therefore be helpful to gain insights into technological advances, its applications, and future challenges for crop improvement.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据