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DEAD-Box RNA Helicases and Genome Stability

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Ddx41 inhibition of DNA damage signaling permits erythroid progenitor expansion in zebrafish

Joshua T. Weinreb et al.

Summary: DEAD-box Helicase 41 (DDX41) plays a critical role in promoting healthy erythropoiesis by protecting the genome from stress, and its mutation may contribute to hematopoietic pathologies.

HAEMATOLOGICA (2022)

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Excessive R-loops trigger an inflammatory cascade leading to increased HSPC production

Joshua T. Weinreb et al.

Summary: Hematopoietic stem and progenitor cells are crucial for blood and immune system maintenance throughout life. The DEAD-box helicase 41 (Ddx41) plays a key role in regulating HSPC production by suppressing the accumulation of R-loops, thus preventing inflammatory signaling pathways and maintaining cellular fitness. This study suggests a possible conservation of mechanism in human cells with decreased DDX41.

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BRCA2 promotes R-loop resolution by DDX5 helicase at DNA breaks to facilitate their repair by homologous recombination

Gaetana Sessa et al.

Summary: BRCA2-deficient cells accumulate DNA-RNA hybrids, and the BRCA2-DDX5 interaction helps in unwinding these structures, enhancing helicase activity and promoting repair of double-strand breaks.

EMBO JOURNAL (2021)

Review Biochemistry & Molecular Biology

DDX3X: structure, physiologic functions and cancer

Jie Mo et al.

Summary: DDX3X, a member of the DEAD-box helicase family, plays critical roles in various stages of RNA metabolism and is implicated in the progression of diseases, especially cancer. Research has gradually unveiled the diverse functions of DDX3X in cancer biology, providing insights into its involvement in tumorigenesis and progression.

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Article Genetics & Heredity

RNA helicase, DDX3X, is actively recruited to sites of DNA damage in live cells

Michael J. Cargill et al.

Summary: Recent studies have shown that DDX3X accumulates at sites of DNA damage in the nucleus rapidly after induction, and its recruitment is mediated by its intrinsically disordered domains, similar to other RNA binding proteins. Inhibition of liquid-liquid phase separation also reduces DDX3X recruitment.

DNA REPAIR (2021)

Review Biochemistry & Molecular Biology

RNA Helicases as Shadow Modulators of Cell Cycle Progression

Olga Sergeeva et al.

Summary: RNA helicases play a crucial role in regulating the activity of key cell cycle regulators at the transcription and translation levels. Some RNA helicases, such as eIF4A, DDX3, and DDX5, have been identified as promising therapeutic targets. However, targeting RNA helicases may lead to side effects, underscoring the importance of caution in their use.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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DDX17 is involved in DNA damage repair and modifies FUS toxicity in an RGG-domain dependent manner

Tyler R. Fortuna et al.

Summary: The study found that DDX17 is significantly downregulated in response to ALS caused by FUS mutations, and restoration of DDX17 levels can suppress FUS-mediated neuropathogenesis and toxicity in vivo.

ACTA NEUROPATHOLOGICA (2021)

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Chemical profiling of DNA G-quadruplex-interacting proteins in live cells

Xiaoyun Zhang et al.

Summary: The CMPP strategy allows for the investigation of DNA G-quadruplex (G4) interactions with proteins in native chromatin. This approach enables the identification of G4-interacting proteins in live cells, providing a chemical strategy to study molecular interactions in cellular chromatin.

NATURE CHEMISTRY (2021)

Article Multidisciplinary Sciences

DHX9-dependent recruitment of BRCA1 to RNA promotes DNA end resection in homologous recombination

Prasun Chakraborty et al.

Summary: DHX9 is a critical player in the repair of DNA damage by homologous recombination, promoting the recruitment of BRCA1 during transcription and facilitating end-resection of DSB.

NATURE COMMUNICATIONS (2021)

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Targeting the DNA damage response in immuno-oncology: developments and opportunities

Roman M. Chabanon et al.

Summary: Immunotherapy has greatly improved cancer treatment outcomes, but many patients still do not benefit from it, partly due to DDR deficiency. DDR-targeted therapies can enhance antitumor immune response by promoting antigenicity, enhancing adjuvanticity, and favoring reactogenicity.

NATURE REVIEWS CANCER (2021)

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UAP56/DDX39B is a major cotranscriptional RNA–DNA helicase that unwinds harmful R loops genome-wide

Carmen Pérez-Calero et al.

GENES & DEVELOPMENT (2020)

Article Biochemistry & Molecular Biology

Novel alternative ribonucleotide excision repair pathways in human cells by DDX3X and specialized DNA polymerases

Valentina Riva et al.

NUCLEIC ACIDS RESEARCH (2020)

Article Biochemistry & Molecular Biology

Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids

Sofiane Y. Mersaoui et al.

EMBO JOURNAL (2019)

Article Genetics & Heredity

Paralog Studies Augment Gene Discovery: DDX and DHX Genes

Ingrid Paine et al.

AMERICAN JOURNAL OF HUMAN GENETICS (2019)

Review Cell Biology

The DNA damage response to transcription stress

Hannes Lans et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2019)

Article Multidisciplinary Sciences

DDX5 helicase resolves G-quadruplex and is involved in MYC gene transcriptional activation

Guanhui Wu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

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Mapping the Ku Interactome Using Proximity-Dependent Biotin Identification in Human Cells

Sanna Abbasi et al.

JOURNAL OF PROTEOME RESEARCH (2019)

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DNA Damage, Liver Injury, and Tumorigenesis: Consequences of DDX3X Loss

Chieh-Hsiang Chan et al.

MOLECULAR CANCER RESEARCH (2019)

Article Multidisciplinary Sciences

DNA damage and genome instability by G-quadruplex ligands are mediated by R loops in human cancer cells

Alessio De Magis et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

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RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination

Claudia Ribeiro de Almeida et al.

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Nucleolus as an emerging hub in maintenance of genome stability and cancer pathogenesis

Mikael S. Lindstrom et al.

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Human proteins that interact with RNA/DNA hybrids

Isabel X. Wang et al.

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DHX9 helicase promotes R-loop formation in cells with impaired RNA splicing

Prasun Chakraborty et al.

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DDX54 regulates transcriptome dynamics during DNA damage response

Miha Milek et al.

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The DEAD-box RNA helicase DDX41 is a novel repressor of p21WAF1/CIP1 mRNA translation

Dominik Peters et al.

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Biochemical Differences and Similarities between the DEAD-Box Helicase Orthologs DDX3X and Ded1p

Deepak Sharma et al.

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DNA-Damage Response RNA-Binding Proteins (DDRBPs): Perspectives from a New Class of Proteins and Their RNA Targets

Martin Dutertre et al.

JOURNAL OF MOLECULAR BIOLOGY (2017)

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Wanted DEAD/H or Alive: Helicases Winding Up in Cancers

Wanpei Cai et al.

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Targeting MicroRNAs in Prostate Cancer Radiotherapy

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An ATR-dependent function for the Ddx19 RNA helicase in nuclear R-loop metabolism

Dana Hodroj et al.

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Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

Corina Ohle et al.

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Targeted inactivation of murine Ddx3x: essential roles of Ddx3x in placentation and embryogenesis

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Roles of RNA-Binding Proteins in DNA Damage Response

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DEAD Box 1 Facilitates Removal of RNA and Homologous Recombination at DNA Double-Strand Breaks

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Multiomic Analysis of the UV-Induced DNA Damage Response

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Inherited and Somatic Defects in DDX41 in Myeloid Neoplasms

Chantana Polprasert et al.

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Targeting DDX3 with a small molecule inhibitor for lung cancer therapy

Guus M. Bol et al.

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Post-Transcriptional Regulation of DNA Damage-Responsive Gene Expression

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Elevated DDX21 regulates c-Jun activity and rRNA processing in human breast cancers

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DDX3 regulates DNA damage-induced apoptosis and p53 stabilization

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MicroRNAs and DNA damage response Implications for cancer therapy

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DEAD box RNA helicase functions in cancer

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