期刊
GENES
卷 12, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/genes12091335
关键词
references genes; real-time quantitative PCR; gene stability; gliomas
资金
- National Institute of Pediatrics, Mexico City, Mexico (Recursos Fiscales para la Investigacion) [INP 031/2018, INP 041/2018]
- Fondos Federales, Mexico [HIM/2019/036 SSA 1595]
- CONACYT fellowship
- CONACyT [549950]
The study evaluated the expression of metabolic pathway-related genes in tumors and identified PKM and TPI1 as two novel genes suitable as reference genes. Results showed that genes involved in metabolic pathways were expressed differently in glioma samples of different CNS WHO grades, indicating a potential model for studying tumor development and finding molecular targets for glioma treatment.
Gliomas are heterogeneous, solid, and intracranial tumors that originate from glial cells. Malignant cells from the tumor undergo metabolic alterations to obtain the energy required for proliferation and the invasion of the cerebral parenchyma. The alterations in the expression of the genes related to the metabolic pathways can be detected in biopsies of gliomas of different CNS WHO grades. In this study, we evaluated the expression of 16 candidate reference genes in the HMC3 microglia cell line. Then, statistical algorithms such as BestKeeper, the comparative Delta C-T method, geNorm, NormFinder, and RefFinder were applied to obtain the genes most suitable to be considered as references for measuring the levels of expression in glioma samples. The results show that PKM and TPI1 are two novel genes suitable for genic expression studies on gliomas. Finally, we analyzed the expression of genes involved in metabolic pathways in clinical samples of brain gliomas of different CNS WHO grades. RT-qPCR analysis showed that in CNS WHO grade 3 and 4 gliomas, the expression levels of HK1, PFKM, GAPDH, G6PD, PGD1, IDH1, FASN, ACACA, and ELOVL2 were higher than those of CNS WHO grade 1 and 2 glioma biopsies. Hence, our results suggest that reference genes from metabolic pathways have different expression profiles depending on the stratification of gliomas and constitute a potential model for studying the development of this type of tumor and the search for molecular targets to treat gliomas.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据