期刊
GENES
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/genes12060807
关键词
Microtus; Msat-160; polymorphism; repeated DNAs; sex chromosomes heterochromatin; telomeric sequences
资金
- Consejeria de Innovacion, Ciencia y Empresa of the Junta de Andalucia [RNM924]
- Universidad de Jaen
- Charles University [PRIMUS/SCI/46, 204069]
The study examined the topology of repetitive DNA elements in the karyotypes of different races of Microtus thomasi and M. atticus, revealing the evolutionary relations and mechanisms shaping the morphology of sex chromosomes. This research used the model of the Microtus thomasi/M. atticus species complex to explore how repetitive DNA elements can contribute to inter-species cytogenetic variability through chromosomal rearrangements.
The voles of the Microtus thomasi/M. atticus species complex demonstrate a remarkable variability in diploid chromosomal number (2n = 38-44 chromosomes) and sex chromosome morphology. In the current study, we examined by in situ hybridization the topology of four satellite DNA motifs (Msat-160, Mth-Alu900, Mth-Alu2.2, TTAGGG telomeric sequences) and two transposons (LINE, SINE) on the karyotypes of nine chromosome races (i.e., populations with unique cytogenetic traits) of Microtus thomasi, and two chromosomal races of M. atticus. According to the topology of the repetitive DNA motifs, we were able to identify six types of biarmed chromosomes formed from either Robertsonian or/and tandem fusions. In addition, we identified 14 X chromosome variants and 12 Y chromosome variants, and we were able to reconstruct their evolutionary relations, caused mainly by distinct mechanisms of amplification of repetitive DNA elements, including the telomeric sequences. Our study used the model of the Microtus thomasi/M. atticus species complex to explore how repetitive centromeric content can alter from chromosomal rearrangements and can shape the morphology of sex chromosomes, resulting in extensive inter-species cytogenetic variability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据