期刊
FRONTIERS IN PHARMACOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2021.719313
关键词
sleep deprivation; autophagy; melatonin receptor 1A; learning and memory; notoginsenoside R1
资金
- National Natural Science Foundation of China [81530096]
- Major International (Regional)Joint Research Project of NSFC [81920108033]
- Shanghai E-Research Institute of Bioactive Constituent in TCM plan
- Opening Project of Shanghai Key Laboratory of Compound Chinese Medicines [17DZ2273300]
- State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University [FAMP2020K]
- Guizhou Science and Technology Platform Talents [QKHRCPT (2019)5106]
- Youth Project of Anhui Natural Science Foundation [2108085QH3720]
This study demonstrates that notoginsenoside R1 (NGR1) extracted from Panax notoginseng can improve impaired learning and memory in sleep deprived mice by reducing excessive autophagy and apoptosis of hippocampal neurons through modulation of the MTNR1A-mediated PI3K/Akt/mTOR signaling pathway.
Sleep deprivation (SD) may cause serious neural injury in the central nervous system, leading to impairment of learning and memory. Melatonin receptor 1A (MTNR1A) plays an important role in the sleep regulation upon activation by melatonin. The present study aimed to investigate if notoginsenoside R1 (NGR1), an active compound isolated from Panax notoginseng, could alleviate neural injury, thus improve impaired learning and memory of SD mice, as well as to explore its underlying action mechanism through modulating MTNR1A. Our results showed that NGR1 administration improved the impaired learning and memory of SD mice. NGR1 prevented the morphological damage and the accumulation of autophagosomes in the hippocampus of SD mice. At the molecular level, NGR1 reversed the expressions of proteins involved in autophagy and apoptosis, such as beclin-1, LC3B, p62, Bcl-2, Bax, and cleaved-caspase 3. Furthermore, the effect of NGR1 was found to be closely related with the MTNR1A-mediated PI3K/Akt/mTOR signaling pathway. On HT-22 cells induced by autophagy inducer rapamycin, NGR1 markedly attenuated excessive autophagy and apoptosis, and the alleviative effect was abolished by the MTNR1A inhibitor. Taken together, NGR1 was shown to alleviate the impaired learning and memory of SD mice, and its function might be exerted through reduction of excessive autophagy and apoptosis of hippocampal neurons by regulating the MTNR1A-mediated PI3K/Akt/mTOR signaling pathway.
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