期刊
FRONTIERS IN PHARMACOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2021.701635
关键词
Alzheimer's disease; beta-asarone; PC12 cells; APPswe; autophagic flux
资金
- Lingnan Medical Research Center of Guangzhou University of Chinese Medicine
Research has shown that beta-asarone can protect PC12 cells from A beta-induced damage by promoting autophagic flux, possibly through enhancing autophagosome-lysosome fusion and/or lysosome function.
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive memory damage and cognitive dysfunction. Studies have shown that defective autophagic flux is associated with neuronal dysfunction. Modulating autophagic activity represents a potential method of combating AD. In Chinese medicine, Acori Tatarinowii Rhizoma is used to treat dementia and amnesia. beta-Asarone, an active component of this rhizome can protect PC12 cells from A beta-induced injury and modulate expression of autophagy factors. However, its cytoprotective mechanisms have yet to be discerned. It is unclear whether beta-asarone affects autophagic flux and, if it does, whether this effect can alleviate A beta cell damage. In the present study, we constructed APPswe-overexpressing PC12 cell line as a cell model of A beta-induced damage and assessed expression of autophagic flux-related proteins as well as the number and morphology of autophagosomes and autolysosomes. Our results show that beta-asarone decreases the expression levels of Beclin-1, p62, LC3-II, and A beta(1-42). beta-Asarone reduced the number of autophagosomes and increased the number of autolysosomes, as determined by confocal laser scanning microscopy and transmission electron microscopy. Our results suggest that beta-asarone can protect PC12 cells from A beta-induced damage by promoting autophagic flux, which may be achieved by enhancing autophagosome-lysosome fusion and/or lysosome function.
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