期刊
FRONTIERS IN MOLECULAR NEUROSCIENCE
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fnmol.2021.715054
关键词
organoids; midbrain; screening; high throughput; automation; toxicity testing
资金
- European Research Council (ERC) [669168]
- International Max Planck Research School-Molecular Biomedicine, Munster, Germany
- European Research Council (ERC) [669168] Funding Source: European Research Council (ERC)
Toxicity testing is essential in the development and approval of chemical compounds, and 3D human organoid systems show promise in providing more relevant models of human physiology. Recent advancements in standardized automated midbrain organoids have allowed for efficient evaluation of toxic effects, with high reproducibility. This study demonstrates the feasibility of quantitatively assessing cell-type-specific toxicity in human organoids in vitro, paving the way for more accurate toxicity predictions.
Toxicity testing is a crucial step in the development and approval of chemical compounds for human contact and consumption. However, existing model systems often fall short in their prediction of human toxicity in vivo because they may not sufficiently recapitulate human physiology. The complexity of three-dimensional (3D) human organ-like cell culture systems (organoids) can generate potentially more relevant models of human physiology and disease, including toxicity predictions. However, so far, the inherent biological heterogeneity and cumbersome generation and analysis of organoids has rendered efficient, unbiased, high throughput evaluation of toxic effects in these systems challenging. Recent advances in both standardization and quantitative fluorescent imaging enabled us to dissect the toxicities of compound exposure to separate cellular subpopulations within human organoids at the single-cell level in a framework that is compatible with high throughput approaches. Screening a library of 84 compounds in standardized human automated midbrain organoids (AMOs) generated from two independent cell lines correctly recognized known nigrostriatal toxicants. This approach further identified the flame retardant 3,3 ',5,5 '-tetrabromobisphenol A (TBBPA) as a selective toxicant for dopaminergic neurons in the context of human midbrain-like tissues for the first time. Results were verified with high reproducibility in more detailed dose-response experiments. Further, we demonstrate higher sensitivity in 3D AMOs than in 2D cultures to the known neurotoxic effects of the pesticide lindane. Overall, the automated nature of our workflow is freely scalable and demonstrates the feasibility of quantitatively assessing cell-type-specific toxicity in human organoids in vitro.
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