期刊
FEBS OPEN BIO
卷 11, 期 8, 页码 2304-2318出版社
WILEY
DOI: 10.1002/2211-5463.13239
关键词
CRISPR; Cas9; proteome; sericin 1; silkworm; transcriptome
资金
- National Natural Science Foundation of China [31772676]
This study successfully disrupted the sericin 1 gene in domestic silkworms using CRISPR/Cas9-mediated gene editing, resulting in the generation of homozygous mutants. The research findings suggest that sericin 1 plays an important role in the cellular immune system and has a synergistic effect with certain protease inhibitors, shedding light on the function of sericin 1 and the mechanism of silk secretion.
The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9-mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation of sericin 1 protein at 323 amino acids. Comparative transcriptomic and proteomic analyses of middle silk gland cells from wild-type individuals and mutants were performed on the fourth day of the fifth instar, and the results suggest that sericin 1 plays an important role in the cellular immune system. In addition, the results suggest that sericin 1 has a synergistic effect with some protease inhibitors and that the secretion of these proteins is strictly regulated. These results will provide new insights into the function and expression pattern of sericin 1 and the mechanism of silk secretion.
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