Real-time Conversion of Electrochemical Currents into Fluorescence Signals Using 8-Hydroxypyrene-1,3,6-trisulfonic Acid (HPTS) and Amplex Red as Fluorogenic Reporters

This paper elaborates on an instrumental scheme for translating electrochemical reactions occurring at an electrode under potentiostatic control into fluorescent signals. We examine the performance enhancement of this device using recently introduced water-soluble fluorescent reporter systems, either the pH-sensitive 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) system or the electrofluorogenic Amplex Red species. The conversion of stationary and transient, cathodic and anodic electrochemical currents into fluorescence is demonstrated. We show that pure capacitive currents can also be converted to fluorescence by our setup, highlighting the rarely observed coupling between a faradaic reaction at one electrode and capacitive charging at another.

Automated summary

This paper discusses an instrumental scheme for translating electrochemical reactions at an electrode into fluorescent signals under potentiostatic control. Using water-soluble fluorescent reporter systems, the device is shown to enhance performance, converting both stationary and transient, cathodic and anodic electrochemical currents into fluorescence. Additionally, it demonstrates the conversion of pure capacitive currents into fluorescence, highlighting the rarely observed coupling between a faradaic reaction at one electrode and capacitive charging at another.