Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity

Recent advances in nanotechnology techniques enable the production of polymeric nanoparticles with specific morphologies and dimensions and, by tailoring their surfaces, one can manipulate their characteristics to suit specific applications. In this work we report an innovative approach for the immobilization of a commercial lipase from Candida rugosa (CRL) which employs nanostructured polymeric carriers conjugated with anti-lipase monoclonal antibodies (MoAbs). MoAbs were chemically conjugated on the surface of polymeric nanoparticles and used to selectively adsorb CRL molecules. Hydrolytic enzymatic assays evidenced that such immobilization technique afforded a significant enhancement of enzymatic activity in comparison to the free enzyme.

Automated summary

Recent advances in nanotechnology have enabled the production of polymeric nanoparticles with specific morphologies and dimensions, allowing for tailored surfaces to manipulate characteristics for specific applications. Utilizing nanostructured polymeric carriers conjugated with anti-lipase monoclonal antibodies, an innovative approach for the immobilization of a commercial lipase from Candida rugosa was developed. Chemical conjugation of MoAbs on polymeric nanoparticles resulted in selective adsorption of CRL molecules, leading to a significant enhancement of enzymatic activity compared to the free enzyme.