期刊
CARTILAGE
卷 13, 期 2_SUPPL, 页码 1583S-1601S出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/19476035211035418
关键词
meniscus cartilage; composite; polycaprolactone; silk fibroin; gelatin
类别
资金
- Shahrekord University of Medical Sciences [3151]
The PCL/SF/Gel/AA composite scaffolds seeded with ASCs showed suitable mechanical properties and high antioxidant capacity, as well as good biocompatibility and proliferation for chondrocytes. The scaffold could successfully improve meniscus healing in damaged rabbits.
Objective Meniscus injuries in the inner avascular zone have weak intrinsic self-healing capacity and often progress to osteoarthritis. This study focused on evaluating the effects of polycaprolactone/silk fibroin/gelatin/ascorbic acid (PCL/SF/Gel/AA) composite scaffolds seeded with adipose-derived mesenchymal stem cells (ASCs), in the meniscus repair. Design To this end, composite scaffolds were cross-linked using N-hydroxysuccinimide and 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride. Scaffolds were then characterized by scanning electron microscope, mechanical tests, total antioxidant capacity, swelling, and toxicity tests. Results The PCL/SF/Gel/AA scaffolds exhibited suitable mechanical properties. Furthermore, vitamin C rendered them the highest antioxidant capacity. The PCL/SF/Gel/AA scaffolds also showed good biocompatibility and proliferation for chondrocytes. Moreover, the PCL/SF/Gel/AA scaffold seeded with allogeneic ASCs was engrafted in New Zealand rabbits who underwent unilateral punch defect in the medial meniscus of the right knee. After 2 months postimplantation, macroscopic and histologic studies for new meniscus cartilage were performed. Conclusions Our results indicated that the PCL/SF/Gel/AA composite scaffolds seeded with allogeneic ASCs could successfully improve meniscus healing in damaged rabbits.
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