4.6 Article

Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copolymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects

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MICROMACHINES
卷 12, 期 7, 页码 -

出版社

MDPI
DOI: 10.3390/mi12070822

关键词

tissue engineering; biomaterial; hyaluronate; mesenchymal stem cells; hydroxyapatite; bone regeneration; gelatin; bone defect

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  1. National Taiwan University Hospital [107-004004]

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This study investigated the use of Gel/HA-HAP and Gel/HA-HAP-SDF-1 composites in enhancing bone regeneration in a bone defect model. The results showed that both Gel/HA-HAP composite groups exhibited better bone generation compared to the control group, with the composite containing SDF-1 showing a trend of faster bone growth. This suggests that Gel/HA-HAP-SDF-1 composites may be a feasible material for filling traumatic bone defects.
Bone defects of orthopedic trauma remain a challenge in clinical practice. Regarding bone void fillers, besides the well-known osteoconductivity of most bone substitutes, osteoinductivity has also been gaining attention in recent years. It is known that stromal cell-derived factor-1 (SDF-1) can recruit mesenchymal stem cells (MSCs) in certain circumstances, which may also play an important role in bone regeneration. In this study, we fabricated a gelatin/hyaluronate (Gel/HA) copolymer mixed with hydroxyapatite (HAP) and SDF-1 to try and enhance bone regeneration in a bone defect model. After material characterization, these Gel/HA-HAP and Gel/HA-HAP-SDF-1 composites were tested for their biocompatibility and ability to recruit MSCs in vitro. A femoral condyle bone defect model of rats was used for in vivo studies. For the assessment of bone healing, micro-CT analysis, second harmonic generation (SHG) imaging, and histology studies were performed. As a result, the Gel/HA-HAP composites showed no systemic toxicity to rats. Gel/HA-HAP composite groups both showed better bone generation compared with the control group in an animal study, and the composite with the SDF-1 group even showed a trend of faster bone growth compared with the composite without SDF-1 group. In conclusion, in the management of traumatic bone defects, Gel/HA-HAP-SDF-1 composites can be a feasible material for use as bone void fillers.

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