期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 19, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12951-021-01000-5
关键词
Hyaluronic acid; Collagen; Nanofiber; M2 macrophage; Hollow organs regeneration
资金
- National Natural Science Foundation of China [82070695, 81900619]
- National Clinical Key Specialist Construction Project (pediatric surgery) [5001-2140019]
- Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease [2019B030301004]
- Postdoctoral Science Foundation of China [2018M630936]
This study demonstrates that hyaluronic acid functionalized collagen nanofibers can effectively modulate the pro-healing expression of macrophages, promoting urethral regeneration. The use of tubular HA-collagen nanofibers significantly facilitates urethral regeneration, with recruited anti-inflammatory M2 macrophages promoting angiogenesis and proliferation of urothelial progenitor cells in the regenerated urethra.
In situ tissue engineering utilizes the regenerative potential of the human body to control cell function for tissue regeneration and has shown considerable prospect in urology. However, many problems are still to be understood, especially the interactions between scaffolds and host macrophages at the wound site and how these interactions direct tissue integration and regeneration. This study was designed to evaluate the efficacy of hyaluronic acid (HA) functionalized collagen nanofibers in modulating the pro-healing phenotype expression of macrophages for urethral regeneration. Tubular HA-collagen nanofibers with HA-coating were prepared by coaxial electrospinning. The formation of a thin HA-coating atop each collagen nanofiber endowed its nanofibrous mats with higher anisotropic wettability and mechanical softness. The macrophages growing on the surface of HA-collagen nanofibers showed an elongated shape, while collagen nanofibers' surface exhibited a pancake shape. Immunofluorescence and ELISA analysis showed that elongation could promote the expression of M2 phenotype marker and reduce the secretion of inflammatory cytokines. In vivo experiments showed that tubular HA-collagen nanofibers significantly facilitate male puppy urethral regeneration after injury. In the regenerated urethra bridged by tubular HA-collagen nanofibers, anti-inflammatory M2 macrophages are recruited to the surface of the scaffold, which can promote angiogenesis and endogenous urothelial progenitor cell proliferation.
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