期刊
GENOME
卷 59, 期 11, 页码 1049-1061出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/gen-2016-0026
关键词
Pan-phylum probes; LATE-PCR; Lights-On/Lights-Off probes; Closed-Tube Barcoding; fluorescent signatures
Here, we present a new approach for increasing the rate and lowering the cost of identifying, cataloging, and monitoring global biodiversity. These advances, which we call Closed-Tube Barcoding, are one application of a suite of proven PCR-based technologies invented in our laboratory. Closed-Tube Barcoding builds on and aims to enhance the profoundly important efforts of the International Barcode of Life initiative. Closed-Tube Barcoding promises to be particularly useful when large numbers of small or rare specimens need to be screened and characterized at an affordable price. This approach is also well suited for automation and for use in portable devices.
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