4.7 Article

New Sources of Eastern Filbert Blight Resistance and Simple Sequence Repeat Markers on Linkage Group 6 in Hazelnut (Corylus avellana L.)

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FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -

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FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.684122

关键词

Anisogramma anomala; Corylus avellana; eastern filbert blight; hazelnut; simple sequence repeat; microsatellite; disease resistance; linkage map

资金

  1. Oregon Hazelnut Commission
  2. Oregon Agricultural Experiment Station
  3. USDA-NIFA Specialty Crops Research Initiative Competitive Grant [2016-51181-25412]
  4. USDA-NIFA Agriculture and Food Research Initiative Competitive Grant [2014-67013-22421]
  5. USDA Specific Cooperative Agreement for Eastern Filbert Blight Research
  6. NIFA [2016-51181-25412, 914305, 687495, 2014-67013-22421] Funding Source: Federal RePORTER

向作者/读者索取更多资源

The commercial production of hazelnut in Oregon's Willamette Valley is threatened by eastern filbert blight (EFB), and genetic resistance is considered the most effective way to control the disease. New SSR markers and resistance sources on LG6 were developed and investigated in this study, providing potential for breeding new cultivars with stacked resistance genes. Further investigation is needed for some resistance sources where SSR marker scores were not correlated with disease response.
Commercial production of hazelnut (Corylus avellana) in Oregon's Willamette Valley is threatened by eastern filbert blight (EFB), a serious canker disease caused by the pyrenomycete Anisogramma anomala (Peck) E. Muller. The fungus also prevents the establishment of hazelnut orchards in eastern North America. Genetic resistance is considered the most effective way to control the disease. A high level of EFB resistance was first discovered in 'Gasaway'. This resistance is conferred by a dominant allele at a single locus on linkage group 6 (LG6). Resistance from several additional sources has been assigned to the same chromosomal region. In this study, new simple sequence repeat (SSR) markers were developed for the resistance region on LG6 and new sources of resistance were investigated. Forty-two new SSR markers were developed from four contigs in the genome sequence of 'Jefferson' hazelnut, characterized, and nine of them were placed on LG6 of the genetic map. Accessions representing 12 new sources of EFB resistance were crossed with susceptible selections resulting in 18 seedling populations. Segregation ratios in the seedling populations fit the expected 1:1 ratio for 10 sources, while one source showed an excess of resistant seedlings and another showed an excess of susceptible seedlings. Based on correlation of disease response and scores of SSR markers in the 'Gasaway' resistance region in the seedlings, eight resistance sources were assigned to LG6. Linkage maps were constructed for each progeny using SSR markers. The LG6 resistance sources include two selections (#23 and #26) from the Russian Research Institute of Forestry and Mechanization near Moscow, four selections from southern Russia, one selection (OSU 1185.126) from Crimea, one selection (OSU 533.129) from Michigan, Corylus heterophylla 'Ogyoo' from the South Korea, and the interspecific hybrid 'Estrella #1'. These new LG6 resistance sources and SSR markers should be useful in breeding new cultivars, including the pyramiding of resistance genes. For the other four resistance sources (Moscow #37, hybrid selection OSU 401.014, C. americana 'Winkler' and C. americana OSU 366.060), SSR marker scores on linkage groups 6, 7 and 2 were not correlated with disease response and merit further investigation.

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