期刊
FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.690095
关键词
grape; resveratrol; transcriptome; ultra performance liquid chromatography-high resolution tandem mass spectrometry; weighted gene co-expression network analysis
资金
- Natural Science Foundation of Jiangsu Province [BK20190899]
- Doctor of Entrepreneurship and Innovation program of Jiangsu Province
- Yangzhou City's Green and Golden Phoenix Program
The study found that bud sport accumulates trans-resveratrol earlier and increases the contents of cis-resveratrol in the earlier stages, but decreases its contents in the later stages. RNA-Seq and WGCNA analysis identified transcripts related to resveratrol metabolism, providing a theoretical basis for genetic improvement of grapes.
Resveratrol is a natural polyphenol compound produced in response to biotic and abiotic stresses in grape berries. However, changes in resveratrol caused by bud sport in grapes are scarcely reported. In this study, trans-resveratrol and cis-resveratrol were identified and quantified in the grape berries of 'Summer Black' and its bud sport 'Nantaihutezao' from the veraison to ripening stages using ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS). We found that bud sport accumulates the trans-resveratrol earlier and increases the contents of cis-resveratrol in the earlier stages but decreases its contents in the later stages. Simultaneously, we used RNA-Seq to identify 51 transcripts involved in the stilbene pathways. In particular, we further identified 124 and 19 transcripts that negatively correlated with the contents of trans-resveratrol and cis-resveratrol, respectively, and four transcripts encoding F3'5'H that positively correlated with the contents of trans-resveratrol by weighted gene co-expression network analysis (WGCNA). These transcripts may play important roles in relation to the synergistic regulation of metabolisms of resveratrol. The results of this study can provide a theoretical basis for the genetic improvement of grapes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据