4.7 Article

RNA Viral Vectors for Accelerating Plant Synthetic Biology

期刊

FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.668580

关键词

RNA viruses; RNA viral vectors; gene editing; synthetic biology; plant synthetic biology; synthetic transcription factors; viral engineering

资金

  1. Defense Advanced Research Projects Agency (DARPA) [HR0011-17-2-0053]
  2. University of Minnesota College of Biological Sciences

向作者/读者索取更多资源

The tools of synthetic biology offer great potential to uncover fundamental mechanisms in plant development and metabolism, but typically require transgenesis. Delivery of foreign genetic material to plants using systemically mobile RNA virus vectors can minimize transgenesis, revolutionizing plant synthetic biology by reducing costs, accelerating experimental timescales, and making genetic manipulation easier.
The tools of synthetic biology have enormous potential to help us uncover the fundamental mechanisms controlling development and metabolism in plants. However, their effective utilization typically requires transgenesis, which is plagued by long timescales and high costs. In this review we explore how transgenesis can be minimized by delivering foreign genetic material to plants with systemically mobile and persistent vectors based on RNA viruses. We examine the progress that has been made thus far and highlight the hurdles that need to be overcome and some potential strategies to do so. We conclude with a discussion of biocontainment mechanisms to ensure these vectors can be used safely as well as how these vectors might expand the accessibility of plant synthetic biology techniques. RNA vectors stand poised to revolutionize plant synthetic biology by making genetic manipulation of plants cheaper and easier to deploy, as well as by accelerating experimental timescales from years to weeks.

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