期刊
FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.723636
关键词
rice blast fungus; conidiation; hyphal growth; gene differential expression; alternative splicing; long non-coding RNAs; premature termination codons
资金
- National Key Research and Development Plan [2016YFD0300703]
- Natural Science Foundation of China [31872916]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1042]
This study identified 3,374 novel genes through RNA-Seq analysis, with the majority likely transcribing lncRNAs, most of which were located in intergenic regions. Additionally, 2,358 genes were found to undergo alternative splicing events. The results suggest that lncRNA transcripts and alternative splicing events are two important mechanisms for regulating gene expression in the rice blast fungus.
Pyricularia oryzae causes the rice blast, which is one of the most devastating crop diseases worldwide, and is a model fungal pathogen widely used for dissecting the molecular mechanisms underlying fungal virulence/pathogenicity. Although the whole genome sequence of P. oryzae is publicly available, its current transcriptomes remain incomplete, lacking the information on non-protein coding genes and alternative splicing. Here, we performed and analyzed RNA-Seq of conidia and hyphae, resulting in the identification of 3,374 novel genes. Interestingly, the vast majority of these novel genes likely transcribed long non-coding RNAs (lncRNAs), and most of them were localized in the intergenic regions. Notably, their expressions were concomitant with the transcription of neighboring genes thereof in conidia and hyphae. In addition, 2,358 genes were found to undergo alternative splicing events. Furthermore, we exemplified that a lncRNA was important for hyphal growth likely by regulating the neighboring protein-coding gene and that alternative splicing of the transcription factor gene CON7 was required for appressorium formation. In summary, results from this study indicate that lncRNA transcripts and alternative splicing events are two important mechanisms for regulating the expression of genes important for conidiation, hyphal growth, and pathogenesis, and provide new insights into transcriptomes and gene regulation in the rice blast fungus.
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