4.7 Article

E183K Mutation in Chalcone Synthase C2 Causes Protein Aggregation and Maize Colorless

期刊

FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.679654

关键词

maize; colorless mutant; ethyl methyl sulfone; flavonoid biosynthesis; MutMap; prokaryotic expression; transient expression

资金

  1. National Key Research and Development Program of China [2016YFD0101203]
  2. National Natural Science Foundation of China [31801372]

向作者/读者索取更多资源

A novel colorless maize mutant was identified and characterized, showing reduced pigmentation throughout its life cycle. The E183K mutation in maize chalcone synthase C2 was identified as the causal mutation for the colorless phenotype. This mutation led to protein aggregation of chalcone synthase, providing insight into protein aggregation in plants.
Flavonoids give plants their rich colors and play roles in a number of physiological processes. In this study, we identified a novel colorless maize mutant showing reduced pigmentation throughout the whole life cycle by EMS mutagenesis. E183K mutation in maize chalcone synthase C2 (ZmC2) was mapped using MutMap strategy as the causal for colorless, which was further validated by transformation in Arabidopsis. We evaluated transcriptomic and metabolic changes in maize first sheaths caused by the mutation. The downstream biosynthesis was blocked while very few genes changed their expression pattern. ZmC2-E183 site is highly conserved in chalcone synthase among Plantae kingdom and within species' different varieties. Through prokaryotic expression, transient expression in maize leaf protoplasts and stable expression in Arabidopsis, we observed that E183K and other mutations on E183 could cause almost complete protein aggregation of chalcone synthase. Our findings will benefit the characterization of flavonoid biosynthesis and contribute to the body of knowledge on protein aggregation in plants.

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