A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.

Automated summary

Virus-induced gene silencing (VIGS) is a promising high-throughput reverse genetic tool in plants, and the review of technological advancements in the widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, requires timely updates. This article mainly reviews viral vector construction, advances in inoculation methods, important influential factors, and recent applications in diverse plant species, providing a better understanding and advice for functional gene analysis related to crop improvements.