期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.649582
关键词
Arthrobotrys oligospora; cell wall integrity pathway; mitogen-activated protein kinases Bck1 and Mkk1; cell wall damage; Woronin body; trap formation
类别
资金
- National Natural Science Foundation of China [31960556, U1402265]
- Applied Basic Research Foundation of Yunnan Province [202001BB050004]
- Yunnan Local Colleges Applied Basic Research Project [2017FH001-030]
The study characterized the role of Bck1 and Mkk1 in the nematode-trapping fungus Arthrobotrys oligospora in regulating conidiation and cell wall synthesis. The results showed that these genes play important roles in cell wall integrity, spore formation, and cell wall biosynthesis.
The cell wall integrity (CWI) pathway is composed of three mitogen-activated protein kinases (MAPKs), Bck1, Mkk1/2, and Slt2, and is one of the main signaling pathways for fungal pathogenesis, cell wall synthesis, and integrity maintenance. In this study, we characterized orthologs of Saccharomyces cerevisiae Bck1 and Mkk1 in the nematode-trapping (NT) fungus Arthrobotrys oligospora by multiple phenotypic comparison, and the regulation of conidiation and cell wall synthesis was analyzed using real-time PCR (RT-PCR). Both Delta AoBck1 and Delta AoMkk1 mutants showed severe defects in vegetative growth, cell nucleus number, and stress resistance. Both the mutants were unable to produce spores, and the transcription of several genes associated with sporulation and cell wall biosynthesis was markedly downregulated during the conidiation stage. Further, cell walls of the Delta AoBck1 and Delta AoMkk1 mutants were severely damaged, and the Woronin body failed to respond to cellular damage. In particular, the mutants lost the ability to produce mycelial traps for nematode predation. Taken together, AoBck1 and AoMkk1 play a conserved role in mycelial growth and development, CWI, conidiation, multi-stress tolerance, trap formation, and pathogenicity. We highlighted the role of AoBck1 and AoMkk1 in regulating the Woronin body response to cellular damage and cell nucleus development in A. oligospora.
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