期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.708280
关键词
Cryptococcus gattii; variant identification; in vitro drug sensitivity; Guangxi; C. neoformansvar.grubii
类别
资金
- National Natural Science Foundation of China [81960567]
- Natural Science Foundation of Guangxi Province of China [2020GXNSFGA238001]
- First Affiliated Hospital of Guangxi Medical University Provincial and Ministerial Key Laboratory Cultivation Project: Guangxi Key Laboratory of Tropical Fungiand Mycosis Research [YYZS2020006]
This study investigated the in vitro drug sensitivity of Cryptococcus spp. from Guangxi, Southern China. The results indicated that most isolates were susceptible to commonly used and novel antifungal drugs, with some C. neoformans var. grubii isolates showing susceptibility-dose dependent to certain drugs. Continuous monitoring of antifungal susceptibility of Cryptococcus isolates is recommended to prevent resistance emergence.
This study analyzed the in vitro drug sensitivity of Cryptococcus spp. from Guangxi, Southern China. One hundred three strains of Cryptococcus were recovered from 86 patients; 14 were HIV positive and 72 were HIV negative. Ninety-two strains were identified as Cryptococcus neoformans var. grubii, while 11 strains were identified as Cryptococcus gattii (5 C. gattii sensu stricto and 6 Cryptococcus deuterogattii). The recovered strains were tested against commonly used antifungal drugs (fluconazole, amphotericin B, 5-fluorocytosine, itraconazole, and voriconazole) and to novel antifungal drugs (posaconazole and isavuconazole) using CLSI M27-A4 method. The results showed that all isolates were susceptible to most antifungal drugs, of which the minimum inhibitory concentration (MIC) ranges were as follows: 0.05-4 mu g/ml for fluconazole, 0.25-1 mu g/ml for amphotericin B; 0.0625-2 mu g/ml for 5-fluorocytosine, 0.0625-0.25 mu g/ml for itraconazole, 0.0078-0.25 mu g/ml for voriconazole, 0.0313-0.5 mu g/ml for posaconazole, 0.0020-0.125 mu g/ml for isavuconazole for C. neoformans var. grubii isolates, and 1-16 mu g/ml for fluconazole, 0.125-1 mu g/ml for 5-fluorocytosine, 0.25-1 mu g/ml for amphotericin B, 0.0625-0.25 mu g/ml for itraconazole, 0.0156-0.125 mu g/ml for voriconazole, 0.0156-0.25 mu g/ml for posaconazole, and 0.0078-0.125 mu g/ml for isavuconazole for C. gattii isolates. Furthermore, some C. neoformans var. grubii isolates were found to be susceptible-dose dependent to 5-fluorocytosine and itraconazole. In addition, a reduction in the potency of fluconazole against C. gattii is possible. We observed no statistical differences in susceptibility of C. neoformans var. grubii and C. gattii in the tested strains. Continuous observation of antifungal susceptibility of Cryptococcus isolates is recommended to monitor the emergence of resistant strains.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据