期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.737248
关键词
adaptive laboratory evolution; microalgae; growth; metabolic regulation; biodegradation of pollutants; stress resistance
类别
资金
- National Natural Science Foundation of China [42077335]
- National Marine Hazard Mitigation Service, Ministry of Natural Resources of the People's Republic of China [2019005AC]
Adaptive laboratory evolution (ALE) experiments are a feasible method for enhancing the phenotype, performance, and stability of microalgae to obtain strains with beneficial mutations. Improving the efficiency of ALE and verifying the stability of resulting strains are the primary challenges that need to be addressed in future research.
Adaptive laboratory evolution (ALE) experiments are a serviceable method for the industrial utilization of the microalgae, which can improve the phenotype, performance, and stability of microalgae to obtain strains containing beneficial mutations. In this article, we reviewed the research into the microalgae ALE test and assessed the improvement of microalgae growth, tolerance, metabolism, and substrate utilization by ALE. In addition, the principles of ALE and the key factors of experimental design, as well as the issues and drawbacks of the microalgae ALE method were discussed. In general, improving the efficiency of ALE and verifying the stability of ALE resulting strains are the primary problems that need to be solved in future research, making it a promising method for the application of microalgae biotechnology.
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