期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2021.614985
关键词
Malaria; Plasmodium vivax; cytoadhesion; bone marrow; gametocytes
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2017/18611-7]
- Conselho Nacional de Desenvolvimento Cient'ifico e Tecnologico (CNPq) [301795/2013-4]
- Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM) (PRO -ESTADO)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- FAPEAM
- FAPESP
- CNPq
In a Plasmodium vivax infection, gametocytes show increased distribution in bone marrow aspirant, indicating the organ's role as a parasite reservoir. Ex vivo assays demonstrate gametocyte adherence to bone marrow endothelial cells, with ICAM1 as a significant receptor and CD36 showing high adhesion rate without significance.
In a Plasmodium vivax infection, it was shown a proportionally increased on gametocyte distribution within the bone marrow aspirant, suggesting a role of this organ as a reservoir for this parasite stage. Here, we evaluated the ex vivo cytoadhesive capacity of P. vivax gametocytes to bone marrow endothelial cells (HBMEC) and investigated the involvement of some receptors in the cytoadhesion process by using transfected CHO cells (CHO-ICAM1, CHO-CD36 and CHO-VCAM), wild type (CHO-K1) or deficient in heparan and chondroitin sulfate (CHO-745). Ex-vivo cytoadhesion assays were performed using a total of 44 P. vivax isolates enriched in gametocyte stages by Percoll gradient in the different cell lines. The majority of isolates (88.9%) were able to adhere to HBMEC monolayer. ICAM1 seemed to be the sole receptor significantly involved. CD-36 was the receptor with higher adhesion rate, despite no significance was noticed when compared to CHO-745. We demonstrated that gametocyte P. vivax adheres ex vivo to bone marrow endothelial cells. Moreover, P. vivax gametocytes display the ability to adhere to all CHO cells investigated, especially to CHO-ICAM1. These findings bring insights to the comprehension of the role of the bone marrow as a P. vivax reservoir and the potential impact on parasite transmission to the vector.
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