期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2021.714162
关键词
characterization; oral and esophageal; microbiota; esophageal precancerous lesions; esophageal squamous cell carcinoma
资金
- National Key Research and Development Program of China [2016YFC1302800, 2016YFC0901402, 2018YFC1313103]
- Beijing Science and Technology Project [D17110002617002]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2016-I2 M-001, 2017-I2 M-1001, 2019-I2 M-2004]
- Sanming Project of Medicine in Shenzhen [SZSM2019110080]
- PUMC Youth Fund
- Fundamental Research Funds for the Central Universities [2017320012]
- PUMC Graduate Innovation Fund [2019100281]
Important evidence suggests that the microbiota plays a key role in esophageal squamous cell carcinoma (ESCC). The study found that with disease progression, the alpha diversity of microbiota in saliva and cell brush samples decreased, and specific bacteria were identified as potential biomarkers. Further research could focus on the functional roles of these characteristic bacteria in ESCC.
Important evidence indicates that the microbiota plays a key role in esophageal squamous cell carcinoma (ESCC). Here, paired saliva and brush specimens were obtained from 276 participants undergoing upper gastrointestinal endoscopic examination before or during screening for upper gastrointestinal (UGI) cancer. The esophageal microbiota was investigated by 16S rRNA gene profiling and next-generation sequencing. We observed that as the disease progressed, the alpha diversity in the saliva and cell brush samples decreased. Linear discriminant analysis effect size (LEfSe) results showed that in both the saliva and cell brush specimens, Granulicatella, Rothia, Streptococcus, Gemella, Leptotrichia and Schaalia were common biomarkers in patients with low-grade dysplasia, Lactobacillus was a common biomarker in patients with high-grade dysplasia, and Bosea, Solobacterium, Gemella, and Peptostreptococcus were common biomarkers in patients with esophageal cancer. The top 3 genera in the saliva and cell brush specimens had areas under the curve (AUCs) of 87.16 and 89.13%, respectively, to distinguish ESCC patients from normal people. The PICRUSt2 results identified in brush samples that patients with ESCC had decreased nitrate reductase functions. Our results suggest that future studies can focus on the function of the characteristic bacteria in ESCC.
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