期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2021.685068
关键词
floR; florfenicol resistance; Pseudomonas aeruginosa; Tn4371; integrative and conjugative elements
资金
- Science and Technology Project of Wenzhou City, China [Y20170205, 2019Y0358]
- Science and Technology Project of Lishui City, China [2017GYX07]
- National Natural Science Foundation of China [81973382, 81960381]
- Natural Science Foundation of Zhejiang Province, China [LQ17H190001, LY19C060002]
- Science & Technology Project of Inner Mongolia Autonomous Region, China [201802125]
The study found different types of floR genes in Pseudomonas aeruginosa, with floR-T1 being the most prevalent. A novel floR variant, floR-T2, showed lower identity with other FloR proteins and could be induced by florfenicol or chloramphenicol. These floR variants play an important role in host resistance and spread, potentially related to the Tn4371 family ICE.
Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host's resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE.
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