期刊
ELIFE
卷 10, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.67436
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资金
- Generalitat Valenciana [APOSTD2018/A113]
- National Institutes of Health [NS103221, NS121273, F31 NS106868, R37 HD032116, R01 NS028478, R01 NS113910, R01 NS091544, R01 NS112357]
- U.S. Department of Veterans Affairs [1I01 BX000252]
- University of California, San Francisco John G. Bowes Research Fund
- Sandler Foundation
By analyzing a single-cell RNA-sequencing dataset of the adult mouse V-SVZ, two distinct populations of NSPCs residing in either the dorsal or ventral V-SVZ were identified. These regional differences in gene expression were validated using a single-nucleus RNA-sequencing reference dataset and immunocytochemistry. The study provides novel markers and territories for understanding the region-specific regulation of adult neurogenesis.
The ventricular-subventricular zone (V-SVZ), on the walls of the lateral ventricles, harbors the largest neurogenic niche in the adult mouse brain. Previous work has shown that neural stem/progenitor cells (NSPCs) in different locations within the V-SVZ produce different subtypes of new neurons for the olfactory bulb. The molecular signatures that underlie this regional heterogeneity remain largely unknown. Here, we present a single-cell RNA-sequencing dataset of the adult mouse V-SVZ revealing two populations of NSPCs that reside in largely non-overlapping domains in either the dorsal or ventral V-SVZ. These regional differences in gene expression were further validated using a single-nucleus RNA-sequencing reference dataset of regionally microdissected domains of the V-SVZ and by immunocytochemistry and RNAscope localization. We also identify two subpopulations of young neurons that have gene expression profiles consistent with a dorsal or ventral origin. Interestingly, a subset of genes are dynamically expressed, but maintained, in the ventral or dorsal lineages. The study provides novel markers and territories to understand the region-specific regulation of adult neurogenesis.
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