期刊
ELIFE
卷 10, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.67681
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资金
- National Institute of Neurological Disorders and Stroke [F99 NS108514, R25 NS076437, R01 NS095994]
- Arizona Biomedical Research Commission [ADHS14-082991]
The study revealed that neurons injected with Toxoplasma are mainly distributed in the cortex and striatum, with abnormal electrophysiology. The injection of Toxoplasma proteins may affect neuronal health and survival, which could be related to the type of neurons.
Toxoplasma gondii is an intracellular parasite that causes a long-term latent infection of neurons. Using a custom MATLAB-based mapping program in combination with a mouse model that allows us to permanently mark neurons injected with parasite proteins, we found that Toxoplasma-injected neurons (TINs) are heterogeneously distributed in the brain, primarily localizing to the cortex followed by the striatum. In addition, we determined that cortical TINs are commonly (>50%) excitatory neurons (FoxP2(+)) and that striatal TINs are often (>65%) medium spiny neurons (MSNs) (FoxP2(+)). By performing single neuron patch clamping on striatal TINs and neighboring uninfected MSNs, we discovered that TINs have highly aberrant electrophysiology. As approximately 90% of TINs will die by 8 weeks post-infection, this abnormal physiology suggests that injection with Toxoplasma protein-either directly or indirectly-affects neuronal health and survival. Collectively, these data offer the first insights into which neurons interact with Toxoplasma and how these interactions alter neuron physiology in vivo.
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