期刊
TOXINS
卷 13, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/toxins13070474
关键词
chronic kidney disease; fraxetin; indoxyl sulfate; alpha-SMA; ERK
资金
- Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation [DTCRD110-I-27, 2420]
Fraxetin has been shown to decrease renal dysfunction and reduce renal interstitial collagen fibers in a mouse model of renal fibrosis, while inhibiting the expression of key proteins involved in fibrosis. This suggests that fraxetin may be beneficial in slowing the progression of chronic kidney disease.
Fraxetin, a natural derivative of coumarin, is known to have anti-inflammatory, anti-oxidant, and hepatoprotective effects in multiple diseases and in liver fibrosis. Whether fraxetin exerts similar effects against renal fibrosis is unknown. In a Unilateral Ureteral Obstruction (UUO) mouse model of renal fibrosis, fraxetin decreased UUO-induced renal dysfunction with a marked reduction in renal interstitial collagen fibers as detected by Masson's Trichrome staining. Fraxetin treatment also inhibited the expression of alpha-SMA, Collagen I, Collagen IV, fibronectin, N-cadherin, vimentin, phosphorylated-ERK, and increased the expression of E-cadherin in UUO mice, as shown by immunohistochemical staining and western blot analysis. In vitro studies showed that fraxetin and indoxyl sulfate had no cytotoxic effects on MES13 kidney cells, but that fraxetin significantly decreased IS-induced cell motility and decreased protein expression of alpha-SMA, N-cadherin, vimentin, and Collagen IV via the ERK-mediated signaling pathway. These findings provide insight into the mechanism underlying fraxetin-induced inhibition of fibrogenesis in renal tissue and suggest that fraxetin treatment may be beneficial for slowing CKD progression.
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