期刊
PLOS NEGLECTED TROPICAL DISEASES
卷 15, 期 7, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pntd.0009549
关键词
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资金
- National Institutes of Health [R21 AI146513-01A1]
- Griswold Endowment Fund
A kdr (410L+V1016I+1534C) allele was found to be the main mechanism of resistance in a pyrethroid-resistant strain of A. aegypti collected in Colombia. The levels of resistance conferred by these mutations varied, with resistance to different pyrethroids ranging from 3.9- to 56-fold. The importance of understanding the levels of resistance conferred by different alleles to different insecticides for pest control and evolution of resistance was discussed.
Aedes aegypti is a primary vector of viral pathogens and is responsible for millions of human infections annually that represent critical public health and economic costs. Pyrethroids are one of the most commonly used classes of insecticides to control adult A. aegypti. The insecticidal activity of pyrethroids depends on their ability to bind and disrupt the voltage-sensitive sodium channel (VSSC). In mosquitoes, a common mechanism of resistance to pyrethroids is due to mutations in Vssc (hereafter referred as knockdown resistance, kdr). In this study, we found that a kdr (410L+V1016I+1534C) allele was the main mechanism of resistance in a pyrethroid-resistant strain of A. aegypti collected in Colombia. To characterize the level of resistance these mutations confer, we isolated a pyrethroid resistant strain (LMRKDR:RK, LKR) that was congenic to the susceptible Rockefeller (ROCK) strain. The full-length cDNA of Vssc was cloned from LKR and no additional resistance mutations were present. The levels of resistance to different pyrethroids varied from 3.9- to 56-fold. We compared the levels of resistance to pyrethroids, DCJW and DDT between LKR and what was previously reported in two other congenic strains that share the same pyrethroid-susceptible background (the ROCK strain), but carry different kdr alleles (F1534C or S989P + V1016G). The resistance conferred by kdr alleles can vary depending on the stereochemistry of the pyrethroid. The 410L+1016I+1534C kdr allele does not confer higher levels of resistance to six of ten pyrethroids, relative to the 1534C allele. The importance of these results to understand the evolution of insecticide resistance and mosquito control are discussed. Author summary Aedes aegypti is a primary vector of viral pathogens and is responsible for millions of human infections and pyrethroids are one of the most commonly used classes of insecticides to control adult A. aegypti. The insecticidal activity of pyrethroids depends on their ability to bind and disrupt the voltage-sensitive sodium channel (VSSC). A common mechanism of resistance to pyrethroids is due to mutations in Vssc (hereafter referred as knockdown resistance mutations, kdr). In this study, we found that a kdr (410L+1016I+154C) allele was the main mechanism of resistance in a pyrethroid-resistant strain of A. aegypti collected in Colombia. We determined the levels of resistance to pyrethroids, DCJW and DDT conferred by this allele. The 410L+1016I+1534C kdr allele does not confer higher levels of resistance to six out of 10 pyrethroids relative to the 1534C allele. Resistance conferred by kdr alleles can vary between pyrethroids and can vary between the stereoisomers. Knowing the levels of resistance conferred by different alleles to different insecticides provides valuable information for pest control and for understanding the evolution of resistance.
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