期刊
GENETICS AND MOLECULAR RESEARCH
卷 15, 期 3, 页码 -出版社
FUNPEC-EDITORA
DOI: 10.4238/gmr.15038431
关键词
Chondrocyte; Tissue engineering; Composite scaffold; Bone matrix; Cartilage; Chitosan
资金
- National Natural Science Foundation of China [81000416]
- Fundamental Research Funds for the Central Universities (Xi'an Jiaotong University)
- Talent Cultivation Fund of the Second Affiliated Hospital of Xi'an Jiaotong University [RC(XM)] [201102]
- Funds for Key Program of the Second Affiliated Hospital of Xi'an Jiaotong University [YJ(ZD)201103, 201302]
- Funds for Shaaxi Province Ke Ji Gong Guan
This study was designed to evaluate bone matrix gelatin (BMG)/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering. Chondrocytes were isolated from costal cartilage of Sprague-Dawley rats and seeded on BMG/fibrin glue or chitosan/gelatin composite scaffolds. After different in vitro culture durations, the scaffolds were subjected to hematoxylin and eosin, Masson's trichrome, and toluidine blue staining, anti-collagen II and anti-aggrecan immunohistochemistry, and scanning electronic microscopy (SEM) analysis. After 2 weeks of culture, chondrocytes were distributed evenly on the surfaces of both scaffolds. Cell numbers and the presence of extracellular matrix components were markedly increased after 8 weeks of culture, and to a greater extent on the chitosan/gelatin scaffold. The BMG/fibrin glue scaffold showed signs of degradation after 8 weeks. Immunofluorescence analysis confirmed higher levels of collagen II and aggrecan using the chitosan/gelatin scaffold. SEM revealed that the majority of cells on the surface of the BMG/fibrin glue scaffold demonstrated a round morphology, while those in the chitosan/gelatin group had a spindle-like shape, with pseudopodia. Chitosan/gelatin scaffolds appear to be superior to BMG/fibrin glue constructs in supporting chondrocyte attachment, proliferation, and biosynthesis of cartilaginous matrix components.
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