期刊
GENETICS
卷 204, 期 2, 页码 645-+出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.115.181750
关键词
MMS21; NSE2; MPH1; nucleolus; RPL19A; Smc5/6; translational stress
The budding yeast E3 SUMO ligase Mms21, also known as Nse2, is a component of the Smc5/6 complex, which regulates sister chromatid cohesion, DNA replication, and repair. Our study shows that the mms21RING Delta mutant exhibits (1) reduced ribosomal RNA production; (2) nuclear accumulation of ribosomal proteins; (3) elevated Gcn4 translation, indicating translational stress; and (4) upregulation of Gcn4 targets. Genes involved in ribosome biogenesis and translation are downregulated in the mms21RING Delta mutant. We identified RPL19A as a novel genetic suppressor of the mms21RING Delta mutant. Deletion of RPL19A partially suppresses growth defects in both smc5-6 and mms21RING Delta mutants as well as nuclear accumulation of ribosome subunits in the mms21RING Delta mutant. Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress. This study suggests that the Smc5/6 complex supports nucleolar function.
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