期刊
GENETICS
卷 203, 期 4, 页码 1679-+出版社
OXFORD UNIV PRESS INC
DOI: 10.1534/genetics.116.190645
关键词
white-opaque switching; transcriptional regulation; transcription networks; transcriptional circuits; Candida albicans
资金
- National Institutes of Health (NIH) [R01-AI049187, R01-AI081704]
- Investigator in the Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund
- Sigma Delta Epsilon fellowship from Graduate Women in Science
- Human Frontier Science Program
- University of California Institute for Mexico and the United States
- Deutsche Forschungsgemeinschaft (DFG) [MO 846/5]
The human fungal pathogen Candida albicans can reversibly switch between two cell types named white and opaque, each of which is stable through many cell divisions. These two cell types differ in their ability to mate, their metabolic preferences and their interactions with the mammalian innate immune system. A highly interconnected network of eight transcriptional regulators has been shown to control switching between these two cell types. To identify additional regulators of the switch, we systematically and quantitatively measured white-opaque switching rates of 196 strains, each deleted for a specific transcriptional regulator. We identified 19 new regulators with at least a 10-fold effect on switching rates and an additional 14 new regulators with more subtle effects. To investigate how these regulators affect switching rates, we examined several criteria, including the binding of the eight known regulators of switching to the control region of each new regulatory gene, differential expression of the newly found genes between cell types, and the growth rate of each mutant strain. This study highlights the complexity of the transcriptional network that regulates the white-opaque switch and the extent to which switching is linked to a variety of metabolic processes, including respiration and carbon utilization. In addition to revealing specific insights, the information reported here provides a foundation to understand the highly complex coupling of white-opaque switching to cellular physiology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据