期刊
INTERNATIONAL JOURNAL OF POLYMER SCIENCE
卷 2021, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2021/5621984
关键词
-
资金
- Zhejiang Provincial Natural Science Foundation of China [LQ20H160017]
- 2019 Jiaxing Key Discipline of Medicine-Clinical Laboratory Diagnostics [2019-cx-03]
EPS1-1 inhibits the proliferation, migration, and invasion of breast cancer MCF-7 cells and promotes apoptosis by inhibiting the activation of the Akt signaling pathway. The expression changes of downstream genes related to proliferation, apoptosis, and migration are affected by EPS1-1 treatment.
Objective. To study the effect of Rhizopus nigricans exopolysaccharide EPS1-1 on the proliferation, apoptosis, and migration of breast cancer MCF-7 cells. Methods. Human breast cancer MCF-7 cells were cultured in vitro and treated with different concentrations of EPS1-1. The effect of EPS1-1 on cell proliferation was tested by the CCK-8 experiment, and the effect of EPS1-1 on cell apoptosis was determined by flow cytometry. And the scratch test was used to detect the impact of EPS1-1 on cell migration. Western blot then was used to measure the expression changes of related proteins in the Akt signaling pathway. Results. Compared with the control group, treatment with EPS1-1 significantly reduced the proliferation, migration, and invasion ability of MCF-7 cells and promoted the apoptosis of MCF-7 cells in a dose-dependent manner. In terms of the underlying mechanism, EPS1-1 can significantly inhibit the phosphorylation of Akt at threonine 308 and serine 473 and cause the expression changes of downstream proliferation-related genes CCND1 and p21, apoptosis-related genes Bcl-2 and Bax, and migration-related genes Vimentin and E-cadherin in terms of their protein levels. Conclusion. EPS1-1 can inhibit the proliferation, migration, and invasion of breast cancer MCF-7 cells and promote the apoptosis of MCF-7 cells by inhibiting the activation of the Akt signaling pathway. Therefore, EPS1-1 can be used as a potential new drug or adjuvant drug for the treatment of breast cancer.
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