期刊
GENETICS
卷 204, 期 3, 页码 1065-+出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.116.194696
关键词
silencing; mating-type switching; long-range regulation; Sas2
资金
- National Institutes of Health [GM-031105]
- National Science Foundation Graduate Research Fellowship [DGE1106400]
In Saccharomyces cerevisiae, a small, intergenic region known as the recombination enhancer regulates donor selection during mating-type switching and also helps shape the conformation of chromosome III. Using an assay that detects transient losses of heterochromatic repression, we found that the recombination enhancer also acts at a distance in cis to modify the stability of gene silencing. In a mating-type-specific manner, the recombination enhancer destabilized the heterochromatic repression of a gene located similar to 17 kbp away. This effect depended on a subregion of the recombination enhancer that is largely sufficient to determine donor preference. Therefore, this subregion affects both recombination and transcription from a distance. These observations identify a rare example of long-range transcriptional regulation in yeast and raise the question of whether other cis elements also mediate dual effects on recombination and gene expression.
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