期刊
CHEMPLUSCHEM
卷 86, 期 9, 页码 1243-1254出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cplu.202100238
关键词
ion-molecule reaction; mass spectrometry; nucleosides; reaction mechanisms; singlet oxygen
资金
- National Science Foundation [CHE 1856362]
This study investigates the reactions of singlet O-2 with the radical cations of 8-oxo-2'-deoxyguanosine and 9-methyl-8-oxoguanine, revealing barrierless, exothermic O-2-addition products and pinpointing the most probable reaction pathway as the C5-terminal O-2-addition. By elucidating the reaction mechanism, kinetics, dynamics, and energetics, this work provides the first report unraveling the synergistic damage of OG by ionizing radiation and singlet O-2.
8-Oxo-2 '-deoxyguanosine (OG) is the most common DNA lesion. Notably, OG becomes more susceptible to oxidative damage than the undamaged nucleoside, forming mutagenic products in vivo. Herein the reactions of singlet O-2 with the radical cations of 8-oxo-2 '-deoxyguanosine (OG(.+)) and 9-methyl-8-oxoguanine (9MOG(.+)) were investigated using ion-molecule scattering mass spectrometry, from which barrierless, exothermic O-2-addition products were detected for both reaction systems. Corroborated by static reaction potential energy surface constructed using multi-reference CASPT2 theory and molecular dynamics simulated in the presence of the reactants ' kinetic and internal energies, the C5-terminal O-2-addition was pinpointed as the most probable reaction pathway. By elucidating the reaction mechanism, kinetics and dynamics, and reaction products and energetics, this work constitutes the first report unraveling the synergetic damage of OG by ionizing radiation and singlet O-2.
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