期刊
CELL REPORTS
卷 36, 期 12, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2021.109746
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资金
- Bill and Melinda Gates Foundation [OPP1168674]
- NIH [5R01AT009562]
- Bill and Melinda Gates Foundation [OPP1168674] Funding Source: Bill and Melinda Gates Foundation
The human microbiota is crucial for host health, with proper development of the infant microbiome being particularly important. Dysbiosis of the microbiome can lead to short-term health issues and long-term disorders. Through the analysis of a metagenomic library constructed from infant stool samples, this study identified bioactive metabolites and previously uncharacterized operons that influence host pathways. This work expands our understanding of microbial effector molecules and their impact on host processes.
The human microbiota plays a critical role in host health. Proper development of the infant microbiome is particularly important. Its dysbiosis leads to both short-term health issues and long-term disorders lasting into adulthood. A central way in which the microbiome interacts with the host is through the production of effector molecules, such as proteins and small molecules. Here, a metagenomic library constructed from 14 infant stool microbiomes is analyzed for the production of effectors that modulate three distinct host pathways: immune response (nuclear factor kappa B [NF-kappa B] activation), autophagy (LC3-B puncta formation), and redox potential (NADH:NAD ratio). We identify microbiome-encoded bioactive metabolites, including commendamide and hydrogen sulfide and their associated biosynthetic genes, as well as a previously uncharacterized autophagy-inducing operon from Klebsiella spp. This work extends our understanding of microbial effector molecules that are known to influence host pathways. Parallel functional screening of metagenomic libraries can be easily expanded to investigate additional host processes.
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