期刊
CELL REPORTS
卷 35, 期 13, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2021.109300
关键词
-
类别
资金
- JST, PRESTO, Japan [JPMJPR18K2]
- NIH [S10 OD018174]
- [26116003]
- [18H02380]
The study reveals that ribosome stalling by the Argonaute-microRNA-SGS3 complex regulates secondary siRNA production in plants. Ribosome stalling is positively correlated with efficient production of secondary siRNAs.
The path of ribosomes on mRNAs can be impeded by various obstacles. One such example is halting of ribosome movement by microRNAs, but the exact mechanism and physiological role remain unclear Here, we find that ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates production of secondary small interfering RNAs (siRNAs) in plants. We show that the double-stranded RNA-binding protein SGS3 interacts directly with the 3' end of the microRNA in an Argonaute protein, resulting in ribosome stalling. Importantly, microRNA-mediated ribosome stalling correlates positively with efficient production of secondary siRNAs from target mRNAs. Our results illustrate a role of paused ribosomes in regulation of small RNA function that may have broad biological implications across the plant kingdom.
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