期刊
CELL REPORTS
卷 36, 期 1, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2021.109335
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资金
- NIH [R01-GM083300, R01-NS083833]
- MSK Core grant [P30-CA008748]
The study revealed that in fruit flies, the behavior of virgin females is regulated by factors such as miR-iab-4/8, Hth, and Dsx, with a double-negative regulatory pathway specifying the virgin behavioral state in the ventral nerve cord.
Virgin females of many species conduct distinctive behaviors, compared with post-mated and/or pregnant individuals. In Drosophila, this post-mating switch is initiated by seminal factors, implying that the default female state is virgin. However, we recently showed that loss of miR-iab-4/8-mediated repression of the transcription factor Homothorax (Hth) within the abdominal ventral nerve cord (VNC) causes virgins to execute mated behaviors. Here, we use genomic analysis of mir-iab-4/8 deletion and hth-microRNA (miRNA) binding site mutants (hth[BSmut]) to elucidate doublesex (dsx) as a critical downstream factor. Dsx and Hth proteins are highly complementary in CNS, and Dsx is downregulated in miRNA/hth[BSmut] mutants. Moreover, virgin behavior is highly dose sensitive to developmental dsx function. Strikingly, depletion of Dsx from very restricted abdominal neurons (SAG-1 cells) abrogates female virgin conducts, in favor of mated behaviors. Thus, a double-negative regulatory pathway in the VNC (miR-iab-4/8. Hth. Dsx) specifies the virgin behavioral state.
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