4.8 Article

Bub1 and CENP-U redundantly recruit Plk1 to stabilize kinetochore-microtubule attachments and ensure accurate chromosome segregation

期刊

CELL REPORTS
卷 36, 期 12, 页码 -

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CELL PRESS
DOI: 10.1016/j.celrep.2021.109740

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资金

  1. National Science Fund for Distinguished Young Scholars [32025011]
  2. National Natural Science Foundation of China (NSFC) [31771499, 32061160470, 31571393, 31322032, 31371359, 31561130155, 32100583, 91953103, 82072855]
  3. Natural Science Foundation of Zhejiang Province [LZ19C070001, LR13C070001, LY17C070003, TY21H160023]
  4. National Key Research and Development Program of China [2017YFA0503602]
  5. Royal Society Newton Advanced Fellowship [NA140075]
  6. China Postdoctoral Science Foundation [2020M681838, 2021M692829]
  7. JSPS KAKENHI [25221106, 17H06167, 16H06279, 15H05972]

向作者/读者索取更多资源

Bub1 and CENP-U play redundant roles in correcting chromosome mis-segregation by recruiting Plk1 to kinetochores to stabilize kinetochore-microtubule attachments. The CENP-O complex does not regulate centromeric localization of Aurora B kinase, leading to sensitivity to Plk1 inhibition but not Aurora B kinase activity upon depletion of Bub1 or CENP-U.
Bub1 is required for the kinetochore/centromere localization of two essential mitotic kinases Plk1 and Aurora B. Surprisingly, stable depletion of Bub1 by similar to 95% in human cells marginally affects whole chromosome segregation fidelity. We show that CENP-U, which is recruited to kinetochores by the CENP-P and CENP-Q subunits of the CENP-O complex, is required to prevent chromosome mis-segregation in Bub1-depleted cells. Mechanistically, Bub1 and CENP-U redundantly recruit Plk1 to kinetochores to stabilize kinetochoremicrotubule attachments, thereby ensuring accurate chromosome segregation. Furthermore, unlike its budding yeast homolog, the CENP-O complex does not regulate centromeric localization of Aurora B. Consistently, depletion of Bub1 or CENP-U sensitizes cells to the inhibition of Plk1 but not Aurora B kinase activity. Taken together, our findings provide mechanistic insight into the regulation of kinetochore function, which may have implications for targeted treatment of cancer cells with mutations perturbing kinetochore recruitment of Plk1 by Bub1 or the CENP-O complex.

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