期刊
GENES CHROMOSOMES & CANCER
卷 56, 期 3, 页码 243-252出版社
WILEY
DOI: 10.1002/gcc.22430
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资金
- Asociacion Espanola Contra el Cancer (AECC - Junta de Barcelona)
- Instituto de Salud Carlos III
- MINECO [PI14/00013, BFU2015-66559-P]
- Red Tematica de Investigacion Cooperativa en Cancer [RD12/0036/0044]
- FEDER
- Sociedad Espanola Hematologia y Hemoterapia
- AGAUR [2014-SGR-225, 2014-SGR-35]
- Deutsche Jose Carreras Leukaemie Stiftung [DJCLS R 14/16, DJCLS AR 14/34]
- Fundacio Internacional Josep Carreras
- Celgene Spain
- Foundation Obra Social La Caixa
- AFM Telethon [AFM 18738]
- European Commission [H2020-MSCA-ITN-2015-675610]
Leukemia cell lines have been widely used in the hematology field to unravel mechanistic insights and to test new therapeutic strategies. Myelodysplastic syndromes (MDS) comprise a heterogeneous group of diseases that are characterized by ineffective hematopoiesis and frequent progress to acute myeloid leukemia (AML). A few cell lines have been established from MDS patients after progression to AML but their characterization is incomplete. Here we provide a detailed description of the immunophenotypic profile of the MDS-derived cell lines SKK-1, SKM-1, F-36P; and MOLM-13. Specifically, we analyzed a comprehensive panel of markers that are currently applied in the diagnostic routine for myeloid disorders. To provide high-resolution genetic data comprising copy number alterations and losses of heterozygosity we performed whole genome single nucleotide polymorphism-based arrays and included the cell line OHN-GM that harbors the frequent chromosome arm 5q deletion. Furthermore, we assessed the mutational status of 83 disease-relevant genes. Our results provide a resource to the MDS and AML field that allows researchers to choose the best-matching cell line for their functional studies. (C) 2016 Wiley Periodicals, Inc.
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