期刊
ADVANCED HEALTHCARE MATERIALS
卷 10, 期 21, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202101141
关键词
cell-laden bioink; disease models; regenerative medicine; 3D bioprinting; vascular tissue
资金
- Texas A&M University Graduate Diversity fellowship
- Texas A&M Engineering Experiment Station (TEES)
- National Institute of Biomedical Imaging and Bioengineering (NIBIB) [DP2 EB026265, R21 EB025945]
- National Science Foundation [CBET 1705852, 1944322]
- Office of the President at Texas AM University
- National Heart Lung and Blood Institute [1R01HL148338, 1R01HL133254, HL133254, 1R01HL157790]
The introduction of a new class of nanoengineered hydrogel-based cell-laden bioinks in 3D bioprinting shows promising potential for replicating the microenvironment of human vasculature, with high printability and cell protection. The ability to print anatomically accurate, multicellular blood vessels provides a valuable tool for modeling vascular function and pathophysiology, demonstrating thromboinflammatory responses similar to in vitro and in vivo models.
3D bioprinting is an emerging additive manufacturing technique to fabricate constructs for human disease modeling. However, current cell-laden bioinks lack sufficient biocompatibility, printability, and structural stability needed to translate this technology to preclinical and clinical trials. Here, a new class of nanoengineered hydrogel-based cell-laden bioinks is introduced, that can be printed into 3D, anatomically accurate, multicellular blood vessels to recapitulate both the physical and chemical microenvironments of native human vasculature. A remarkably unique characteristic of this bioink is that regardless of cell density, it demonstrates a high printability and ability to protect encapsulated cells against high shear forces in the bioprinting process. 3D bioprinted cells maintain a healthy phenotype and remain viable for nearly one-month post-fabrication. Leveraging these properties, the nanoengineered bioink is printed into 3D cylindrical blood vessels, consisting of living co-culture of endothelial cells and vascular smooth muscle cells, providing the opportunity to model vascular function and pathophysiology. Upon cytokine stimulation and blood perfusion, this 3D bioprinted vessel is able to recapitulate thromboinflammatory responses observed only in advanced in vitro preclinical models or in vivo. Therefore, this 3D bioprinted vessel provides a potential tool to understand vascular disease pathophysiology and assess therapeutics, toxins, or other chemicals.
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