期刊
ADVANCED HEALTHCARE MATERIALS
卷 10, 期 16, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202001716
关键词
biosensors; cardiac tissues; contractile forces; gelatin microspheres; microfluidics
资金
- National Natural Science Foundation of China [31627801, 51861145307, 61901412]
- Key Research Project of Zhejiang Province [2019C03066]
- Natural Science Found of Zhejiang Province [LGF19H180022]
The developed method utilizes elastic gelatin microspheres and fluorescent beads to quantify compressive stress in cardiac tissues, allowing for the local quantification of contractile force variation in 3D cardiac tissues. This approach has significant applications in studying mechanical heart failure and drug screening.
Quantifying cardiac contractile force is of paramount important in studying mechanical heart failure and screening therapeutic drugs. However, most existing methods can only measure the in-plane component of twitch force of cardiomyocytes, such that mismatching the centripetal compressive stress of heart beating in physiology. Here, a non-destructive method is developed for quantifying the compressive stress and mapping the distribution of the local stress within the 3D cardiac tissues. In detail, elastic gelatin microspheres labeled with fluorescence beads are fabricated by microfluidic chips with high throughput, and they serve as built-in pressure sensors which are wrapped by cardiomyocytes in 3D tissues. The deformation of microspheres and the displacements of fluorescent beads induced by the contraction of cardiomyocytes are demonstrated to characterize the amount and distribution of the centripetal compressive stress. Further, the method shows a potent capability to locally quantify contractile force variation of 3D cardiac tissues, which is induced by agonist (norepinephrine) and inhibitor (blebbistatin). On the whole, the method significantly improves the 3D measurement of mechanical force in vitro and provides a solution for locally quantifying the compressive stress within engineered cardiac tissues.
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