4.7 Article

Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins

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Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase-mediated cassette exchange system

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Summary: Cell line development through targeted integration (TI) offers a more controlled and efficient method compared to random integration (RI), with the aim of integrating the antibody-expressing cassette to a predetermined site by recombinase-mediated cassette exchange (RMCE). This study describes the establishment of two CHO-K1-M derived TI host cells using the Cre/Lox RMCE system, which demonstrate higher productivity, better stability, and fewer sequence variant occurrences compared to RI cell lines. Additionally, an innovative two-plasmid based RMCE strategy is implemented by inserting a third incompatible LoxP site in the GFP landing pad.

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