Methylene blue can act as an antidote to pesticide poisoning of bumble bee mitochondria

The population of bumble bees and other pollinators has considerably declined worldwide, probably, due to the toxic effect of pesticides used in agriculture. Inexpensive and available antidotes can be one of the solutions for the problem of pesticide toxicity for pollinators. We studied the properties of the thiazine dye Methylene blue (MB) as an antidote against the toxic action of pesticides in the bumble bee mitochondria and found that MB stimulated mitochondrial respiration mediated by Complex I of the electron transport chain (ETC) and increased respiration of the mitochondria treated with mitochondria-targeted (chlorfenapyr, hydramethylnon, pyridaben, tolfenpyrad, and fenazaquin) and non-mitochondrial (deltamethrin, metribuzin, and penconazole) pesticides. MB also restored the mitochondrial membrane potential dissipated by the pesticides affecting the ETC. The mechanism of MB action is most probably related to its ability to shunt electron flow in the mitochondrial ETC.

Automated summary

The study found that Methylene blue can act as an antidote to alleviate the toxic effects of pesticides on bumble bee mitochondria by stimulating mitochondrial respiration, increasing respiration, and restoring mitochondrial membrane potential. The mechanism of action is likely related to its ability to shunt electron flow in the mitochondrial electron transport chain.